"Ozone therapy for breast cancer: It just makes common sense." Veronique Desaulniers, DC.
Dedicated to the Memory of Frederick Sweet, PHD.
Dr. Frederick Sweet's brilliant, imaginative formative research back in 1980 on the effects of ozone on cancer cells, including breast cancer cells, showed some promising results. Unfortunately, Dr. Sweet and his team were unable to continue their critical research. (See Dr. Sweet's study in this section.)
EDITORS' NOTE: THERE ARE MANY WAYS TO USE OZONE. PLEASE CONSULT WITH A KNOWLEDGEABLE MEDICAL PROFESSIONAL BEFORE PURCHASING OR USING ANY OZONE DEVICE!
FEBRUARY 2020: DR. ROBERT ROWEN VIDEO STAGE 4 BREAST CANCER PATIENT IN EXTREME PAIN FROM BONE METS QUITS HER DRUGS WITH INTRAVENOUS TREATMENT AND OZONE
CIRCA SIX MINUTES: https://www.youtube.com/watch?v=R3OO0LAh4CQ&t=103s
*UPDATE: April 2018: - Circa 1 minute Dr. Robert Rowen Video: The Stage 4 Breast Cancer Patient's markers are now all normal! https://www.youtube.com/watch?v=vXChY6PfTq4
December 2017 - Circa 4 Minute Dr. Robert Rowen Video: Stage 4 Breast Cancer Patient Responds to Integrative Care - Including Ozone https://www.youtube.com/watch?v=kbQ6kj-tmWY
HAVE STAGE 1- 3 BREAST CANCER? 2017 STUDY: OZONE THERAPY SIGNIFICANTLY BRINGS DOWN SERUM TK-1 (ENZYME HIGH IN FAST-DIVIDING CANCER CELLS) LEVELS, INHIBITS THE PROGRESSION OF TUMOR CELLS IN BREAST CANCER PATIENTS
After surgery to remove their tumors, 104 breast cancer patients, with varying stages of breast cancer, embarked upon ozone therapy.
The breast cancer patients' levels of TK-1 were tested before and after three sessions of ozone therapy. (Note that TK-1 -Thymidine Kinase-1 - is an enzyme that is associated with cell division. TK-1 is low in resting cells, while more TK-1 is released into the blood during the rapid division of cancer cells.)
Results? "Ozone therapy can effectively reduce the TK1 level of breast cancer patients after resection, inhibit the proliferation of tumor cells, control the disease progression, and it is more significant for patients with stage I to III."
(See Qin Zilin et al., Changes of Serum TK-1 Level in Breast Cancer Patients with Resection After Ozone Therapy, Journal of Modern Oncology, 2017.)
"A recent study compared the changes of serum TK1 levels in patients of breast cancer with resection after ozone therapy and the relationship between the changes of TK1 and tumor stage. Researchers found a significant difference between the levels before and after ozone. They concluded that ozone therapy can effectively reduce the TK1 level of breast cancer patients after resection, inhibit the proliferation of tumor cells, control the disease progression and it is more significant in patients with stage 1 to 3.
Ozone therapies can be valuable adjunctive cancer care therapy and can be done in conjunction with chemo and radiation. Ozone therapy increases oxygen utilization allowing oxygen into every cell. Cancer hates oxygen and ozone kills cancer cells. Ozone has been proven to reduce ROS or reactive oxygen species which cause the formation of free radicals. Patients can choose to do a series of I.V. ozonated saline treatments and glutathione on alternate days. Glutathione is a powerful anti-oxidant that kills cancer cells.
Chemotherapy and radiation cause oxidative stress and a host of side effects. Several cases published in the German literature show that ozone therapy combined with standard oncology produced positive effects, decreased side effects and created less resistance to chemotherapeutic drugs. While chemo and radiation might be effective in individual cases it is not necessarily safe or affordable. Two of our every 5 patients who follow standard of care for cancer get diagnosed with a secondary cancer. Ozone therapy is safe, effective and affordable. Ozone does not cause cancer."
Oxygen has TWO atoms of oxygen, while ozone has THREE atoms of oxygen. Starting with the potential of ozone therapy...
J.D., a friend of Breast Cancer Study and Support, had an MAH (major autohemotherapy) treatment, which entails taking some blood out, ozonating it, and returning it to the body.
J. D. described her blood as looking like "dark wine red" when it was being removed.
J. D. described her blood as looking "cherry red" - fully oxygenated - when it was returned to her body following the ozonationprocedure.
*An interview with ozone practitioner, Dr. Isadora Guggenheim, December 2015.
THE EDITORS INTERVIEWED ISADORA GUGGENHEIM, ND, RN, MS, CNS LMT. ON OZONE AND BREAST CANCER
1. Dr. Guggenheim, if a breast cancer patient has had surgery to remove her tumor, and has declined chemo and radiation, what kinds of ozone therapy do you offer? (Dr. Hulda Clark recommended whole body ozone bagging, even bagging of the affected breast.) And how many ozone treatments does a breast cancer patient typically have?
"We offer all standard kinds of ozone plus more. Patients undergoing treatment are advised about clinical nutrition, cancer-fighting oral nutraceuticals, hydrotherapy and homeopathy
We offer high dose MAH major autohemotherapy, vaginal, rectal and bladder insufflations, limb bagging, add-ons including: glutathione, Vitamin C - low, moderate and high dose, homeopathic nosodes specific for breast cancer and other cancers too. Our I.V. ozone therapy is highly effective as we can deliver up to 750 ml of ozone safely at different gamma ranges from 20 to 86 and above. We offer white and red ozone treatments. We begin with 250 ml of ozonated saline loaded with individualized homeopathic injectables followed by blood drawing with heparin and finally ozonating the patient's drawn blood. Treatments take about an hour. During our I.V. treatment patients receive ozone inhalation because diseases and medicines cross the blood brain barrier. I am not aware of any other clinic offering this full service package. We follow all American Academy of Ozone Therapy guidelines. All patient must go through an intake and be seen by our doctors before they receive I.V. therapies.
Cancer is a systemic disease so I would not choose limb bagging as a frontline treatment. I.V. ozone is much more effective followed by ozone colonics and or ozone sauna. There is no set number of treatments and treatment reflects the progression of the disease. As an average, most do 10-12 MAH treatments before repeating labs and imaging. Then they move on to high dose Vitamin C drips."
2. Dr. Guggenheim, if a person does decide to do chemo and/or radiation, do you do ozone therapy at the same time as the breast cancer patient is undergoing treatment?
"Those patients who choose a hybrid model do weekly or biweekly ozone treatments as ozone is not contraindicated during chemo and radiation. Our treatment protects them from severe side effects down to a cellular level. Chemo and radiation cause DNA damage which we protect with specific homeopathic formulas."
3. Dr. Guggenheim, what kinds of supplements do you feel are most important to complement ozone therapy for the breast cancer patient?
"Outside of radical dietary changes and daily juicing, I recommend high quality high dose nutraceuticals like Turmeric, Broccoli seed extract, mushroom extracts, Resveratrol, probiotics and beta-glucans. There are more, but those are certainly foundational medicines."
4. What is the best way for a breast cancer patient, who would like to pursue ozone therapy, to get ahold of you?
"Cancer cells meet their energy needs by fermentation, not oxidation. Thus, they are dependent upon glucose, and a high oxygen environment is toxic to them." Otto Warburg, MD, 1966.
First, a 2017 oxygen study .... Low oxygen is a "negative prognostic factor" in breast cancer. So, how about using a high dose of oxygen...?
In the 2017 Yttersian S et al. study, the researchers explored whether employing HBOT (hyperbaric oxygen therapy - high oxygen under pressure) would be helpful in the regulation of tumor growth and metastasis in breast cancer.
For their study, the researchers used human breast cancer xenografts in mice. What are xenografts? Xenografts are human breast cancer cells - in this case, both triple positive and triple negative breast cancer cells - that were grafted (injected) in mice.
Will HBOT help reduce tumor growth?
After the mice had HBOT treatments for 90 minutes every third day until the end of the experiment, and "despite the fact that the behavior and prognosis of the triple positive and negative subtypes are different", there was a "similar suppressive effect on tumor growth".
Was mestastasis reduced in the mice?
Although there was an "old perception that it (HBOT) would facilitate tumor growth and spread", affter 16 sessions of 90 minutes of HBOT in a xenograft model of triple negative breast cancer in mice, there was a significant reduction in the "number and area" of metastatic lesions.
CONCLUSION HBOT has a "strong and similar primary tumor suppressive effect" in triple positive and triple negative breast cancer. Import? Both breast cancer tumor types "share a common oxygen dependent and tumor mechanism"
(See Yttersian S et al., Oxygen-Dependent Regulation of Tumor Growth and Metastasis in Human Breast Cancer Xenografts, PLOS One, 2017.)
OXYGEN BACKGROUND: BREAST CANCER TUMORS HAVE REGIONS OF LOW OXYGEN
" ...The prognostic significance of reduced oxygen levels in primary breast tumors of cancer patients is well recognized."
In the Gilkes DM et al. 2013 study, the researchers stated, "Human breast tumors contain regions of hypoxia (low oxygen) in which cells that are located far from a functional blood vessel have significantly reduced oxygen concentrations when compared with normal mammary tissue."
In fact, the researchers observed that "the mean partial pressure of oxygen in breast tumors ranges from 2.5 to 28 mm of mercury, with a median value of 10 mm mercury, as compared with 65 mm mercury in normal breast tissue. Intratumoral partial pressure of oxygen values of <10 mm mercury have been associated with an increased risk of metastasis and mortality."
Why is there low oxygen in a breast cancer tumor?
"Hypoxia within a solid tumor arises from an increase in oxygen utiliztion due to an increase in rapidly dividing cancer cells, and a decrease in oxygen availability due to structurally and functionally abnormal vessels that form within solid tumors. Oxygen availability decreased as distance from the nearest blood vessel increases."
So, what happens when there is low oxygen?
"Breast cancer cells adapt to hypoxic conditions by increasing levels of hypoxia-inducible factors (HIFs), which induce the expression of multiple genes involved in angiogenesis, glucose utilization, resistance to oxidative stress, cell proliferation, resistance to apoptosis, invasion, and metastasis. Breast cancer patients with increased HIF expression levels in primary tumor biopsies are at increased risk of metastasis."
These hypoxia inducible factors appear "to play a role in every step of the metastasis cascade". And, "HIF-1a expression has been implicated as an independent predictor of poor outcome for breast cancer patients."
Thus, it is known that these hypoxia-inducible factors are involved in metastasis. But, in order to metastasize, the cells must be able to go into a new environment (some organ), and be able to survive. Not so easy. In fact, for circulating tumor cells, "... only a small subset for micrometastases and only a subset of these micrometastases form vascularized macrometastases. Cancer cells that have survived and have been reprogrammed by hypoxic conditions have a survival advantage at distant sites." The clinical evidence is that having high HIF-1a levels will have a "greater likelihood of developing metastatic relapse and a shorter survival time".
(See Gilkes DM et al., Role of Hypoxia-Inducible Factors in Breast Cancer Metastasis, Future Oncol, 2013.)
2019 Review Study: (Note: One of the researchers, Dr. Greg Semenza, won the 2019 Nobel Prize for his work on oxygen.)
LOW OXYGEN IN BREAST TUMORS MAY CAUSE INDUCTION OF BREAST CANCER STEM CELLS AND SUBSEQUENT DISEASE PROGRESSION. From the Study: "Clinical studies have revealed that breast cancers contain regions of intratumoral hypoxia (reduced oxygen availability), which activates hypoxia-inducible factors (HIFs). The relationship between intratumoral hypoxia, distant metastasis and cancer mortality has been well established. A major mechanism by which intratumoral hypoxia contributes to disease progression is through induction of the breast cancer stem cell (BCSC) phenotype. BCSCs are a small subpopulation of cells with the capability for self-renewal. BCSCs have been implicated in resistance to chemotherapy, disease recurrence, and metastasis."
(See Xiang L and Semenza GL, Hypozis-Inducible Factors Promote Breast Cancer Stem Cell Specification and Maintenance in Response to Hypoxia or Cytotoxic Chemotherapy, Adv Cancer Res, 2019)
* *ROCKING AND ROLLING CANCER CELLS?
WELL, HOW ABOUT JUST "ROCK1 AND RhoA"?
OXYGEN BACKGROUND: LOW OXYGEN AREAS IN BREAST CANCER TUMORS CREATE HYPOXIA-INDUCIBLE FACTORS THAT TRIGGER PROTEINS THAT HELP BREAST CANCER CELLS GROW "HANDS" AND MOVE AND METASTASIZE!
"...Low oxygen levels within cancer cells trigger the increased production of two proteins—RhoA and ROCK1. These proteins are known to give cancer cells movement and the ability to easily spread."
2022 - A Digression: MORE ROCK AND ROLL - ROCK1 and RHOA WITH INTRATUMOR BACTERIA
Tumor-Resident Intracellular Microbiota Promotes Metastatic Colonization in Breast Cancer, Fu A et al., Cell, April 2022
The thrust of this study is that depleting tumor bacteria will impede metastasis. The depletion of intratumor bacteria reduced metastasis without affecting the primary tumor growth. During metastatic colonization, which is a difficult feat, intratumor bacteria that have hijacked the circulating cancerous tumor cells promote host-cell survival by enhancing resistance to fluid shear stress by reorganizing actin cytokeleton. Indeed, the cancer cells were able to enhance their strength to thwart this stress. But, how? "Intracellular bacteria affect the cancer cells’ RhoA-ROCK pathway, which in turn inhibits the cytoskeleton reorganization that cells usually undergo in response to mechanical stress."
Tumor-resident microbiota, albeit at a low biomass, play an important role in promoting cancer metastasis. Indeed, quite amazingly, the researchers experimented with ONLY ONE INJECTION OF BACTERIA INTO THE TUMOR TISSUE AND CAUSED A TUMOR THAT RARELY METASTASIZES TO BECOME METASTATIC.
SO, WHAT BACTERIA? There were 100 colony-forming units in normal tissue, and 297 colony-forming units in the breast cancer tumor. Specifically, the enriched travelling bacteria were Streptococcus, Lactobacillus, Staphylococcus, and Enterococcus.
HIGHLIGHTS *Conserved intracellular bacterial profile is detected in murine and human breast cancer. *Perturbation of intracellular bacteria induces metastasis but not primary tumor growth. *Intracellular bacteria reorganize actin cytokeleton in circulating tumor cells. *Intracellular bacteria promote circulating tumor cell survival by enhancing resistance to mechanical stress. Not to be excluded are the gut microbiome and immune system that could singly or united play a role with the bacteria in cancer progression
"Caption: In low oxygen conditions, breast cancer cells form structures that facilitate movement, such as filaments that allow the cell to contract (green) and cellular ‘hands’ that grab surfaces to pull the cell along (red)." Credit: Daniele Gilkes.
The Gilkes DM et al. study cited above found not only that breast tumors may have areas of low oxygen, but that cells that are re-programmed in low oxygen in the primary breast tumor have a survival advantage at a distant metastatic site.
But how did these hardy breast cancer cells actually physically move, make it to the metastatic site?
It was another Gilkes DM et al. 2014 study that found that in areas of low oxygen in the primary tumor, hypoxia-inducible factors triggered RhoA and ROCK1 proteins that changed the shape of the breast cancer cells, allowing them to grow "hands" to help in their metastatic voyage.
In the Johns Hopkins Press Release, the prominent hypoxia researcher and team member, Gregg Semenza, MD, commented, “ 'The lack of oxygen activates the hypoxia-inducible factors, which are master control proteins that switch on many genes that help cells adapt to the scarcity of oxygen.' He explains that, while these responses are essential for life, hypoxia-inducible factors also turn on genes that help cancer cells escape from the oxygen-starved tumor by invading blood vessels, through which they spread to other parts of the body."
Long known is that high levels of the proteins, RhoA and ROCK1, which worsen a breast cancer patient's prognosis, give the tumor cells the ability to move. "What the study showed is that low oxygen conditions, which are frequently present in breast cancers, serve as the trigger to increase the production of RhoA and ROCK1 through the action of hypoxia-inducible factors." (Note that the hypoxia-inducible factors actually bind to RhoA and ROCK1.) And, " 'As tumor cells multiply, the interior of the tumor begins to run out of oxygen because it isn't being fed by blood vessels, ' ” Dr. Semenza stated. Hypoxia-inducible factors not only help the cells to adapt to the low oxygen, but the hypoxia factors help the tumor cells to escape through the blood vessels."
And here is a description of these treacherous breast cancer cells that have been endowed by Rho A and ROCK1 with the ability to move....
"Thin, parallel filaments form throughout the cells, allowing them to contract and cellular 'hands' arise, allowing cells to 'grab' external surfaces to pull themselves along."
Plus, it was even "found that the (tumor) cells were much more mobile in the presence of low levels of oxygen than at physiologically normal levels. They had three times as many filaments and many more 'hands' per cell". Chilling structures! (For the study, see Gilkes DM et al., Hypoxia-Inducible Factors Mediate Coordinated RhoA-ROCK1 Expression and Signaling in Breast Cancer Cells, Proc Nat Sci Acad Sci, 2014 ... For the Johns Hopkins Press Release, see http://www.hopkinsmedicine.org/news/media/releases/rock_and_rho_proteins_that_help_cancer_cells.)
* 2016 STUDY - HYPOXIA REGULATES STEMNESS IN HORMONE-NEGATIVE BC CELLS
In their 2016 study, Xie J et al. explored hypoxia and breast cancer cell stemness in hormone-negative breast cancer cells.
"Self-renewal is a fundamental attribute of CSCs (cancer stem cells) and has been demonstrated in xenograft animal experiments and colony-formation assays. Colony-formation assays reveal cells with the proliferative activity to form clones, such that the colony-formation rate reflects the proportion of cells with stemness.
In the present study, we showed that hypoxia significantly increased the colony-formation rate and therefore the proportion of cells with stemness properties. We also measured hypoxia-induced changes in the percentage of BCSCs in MDA-MB-231 (estrogen-negative) cells using flow cytometry analysis. Our results further confirmed the ability of hypoxia to increase the percentage of BCSCs in MDA-MB-231 cells and facilitate stemness transformation. ...Hypoxia is a major feature of solid tumors and exerts multiple effects on the biological behavior of tumor cells. Our experiments showed that hypoxia had no significant cytotoxic effect, but did inhibit apoptosis and regulate stemness in cancer cells. Hypoxia is a major feature of solid tumors and exerts multiple effects on the biological behavior of tumor cells. Our experiments showed that hypoxia had no significant cytotoxic effect, but did inhibit apoptosis and regulate stemness in cancer cells"
The researchers encourage exploring "hypoxia as a mechanism which might not be underestimated in chemo-resistant breast cancer treatment".
Xie J et al., Hypoxia Regulates Stnemness of Breast Cancer MDA-MS-231 Cell, Medical Oncol, 2016
OXYGEN BACKGROUND: THE 2015 HATFIELD TEAM STUDY - BREATHE EXTRA OXYGEN PLUS USE SOME "SUPER-CAFFEINE" PRODUCT FOR ANTI-TUMOR EFFECTS IN MICE? REGRESSION OF SPONTANEOUS METASTASIS FROM ORTHOTOPICALLY (GRAFTED) GROWN BREAST TUMORS?"
Respiratory Hyperoxia Improves Tumor Regression in Preclinical Models of Immunotherapies "Hyperoxia facilitates the infiltration of adoptively transferred T cells into 11-day established pulmonary tumors. .... From B: The average infiltration from -100 tumors was 249 cells/mm2 in control mice and 723 cells mm2 in mice breathing 60% oxygen. From C: Breathing as low as 40% oxygen results in pulmonary tumor regression. From D: Alternating between breathing 60 and 40% oxygen or 60 and 21% oxygen every 12 hours enables tumor regression compared to mice continuously breathing 21% oxygen. Breathing 60% oxygen continuously (24 hours/day) causes the strongest antitumor activity."
Add oxygen? ....... Yes, according to the Hatfield S et al. 2015 study.
Extra oxygen along with wake-up "super-caffeine" (an antagonist of the A2A adenosine receptor) shrinks tumors!
Wouldn't just giving extra oxygen work?
Yes ..... Yes, improving the oxygen to oxygen-starved areas in the tumor will allow the immune cells to enter....
But, in areas where there is low oxygen, cancerous tumors have a nasty trick, namely a compound, adenosine, to put the tumor killer cells to sleep when they come to try to kill the cancer cells. Maybe the reason that the anti-tumor effects of oxygen alone are not always successful could be that there is still some residual influence of adenosine on the immune system. Thus, extra oxygen PLUS supplementing with an anti-adenosine compound are needed for stronger anti-tumor effects.
Laura Neergaard, in a 2015 article in Bioscience Technology, wrote, "Tumors can grow so rapidly that they outpace their blood supply, creating a low-oxygen environment. The lack of oxygen in turn spurs cancer cells to produce a molecule called adenosine, which essentially puts nearby tumor fighters called T cells and natural killer cells to sleep, explained pharmacologist (and co-author) Edwin Jackson." .... The researchers wondered if "just getting more oxygen to an oxygen-starved tumor could strip away that defense. So they put mice with different kinds of lung tumors inside chambers that mimic what's called supplemental oxygen therapy. Air is about 21 percent oxygen, but hospitals can give patients concentrations of 40 percent to 60 percent through masks to treat various disorders. The extra oxygen changed the tumor's environment so that immune cells could get inside and do their jobs...." In a Science Daily article, the long-time prominent oxygen researcher, Michail Sitkovsky, PHD,, a member of the team, stated, "The anti-tumor effects of supplemental oxygen can be further improved by the natural antagonist of the A2A adenosine receptor, which happens to be the caffeine in your coffee. People drink coffee because caffeine prevents the A2A adenosine receptor in the brain from putting us to sleep." Barry Krans, in a 2015 article, summarized researcher Michail Sitkovsky PHD's comments that "delivering 60 percent oxygen - the amount used in hospitals - could improve tumor rejection by flipping the tumor environment from hostile to permissive." As reseacher Sitkovsky stated, " 'The solution was embarrassingly simple. ' " HATFIELD (2015) EDITORS' SUMMARY EDITORS: "Tumors often express unusual antigens and are surrounded by immune cells. Unfortunately, this immune surveillance is imperfect and does not always prevent the tumors from growing. In addition, tumors are often hypoxic, because their rapid growth outstrips that of their blood and oxygen supply. Now, Hatfield et al. have linked these two phenomena by demonstrating that T cells avoid going into the hypoxic areas of tumors. The authors have also shown a way to overcome this problem in mice with lung tumors by having the animals breathe supplementary oxygen. Having a higher concentration of oxygen throughout the body improved the oxygenation inside the tumors, allowing immune cells to enter the tumors and attack them, extending the animals' survival." FROM THE STUDY: Extra oxygen decreases low oxygen in tumors as well as concentrations of extracellular adenosine. The study also shows that this extra oxygen reverses hypoxia-adenosinergic immunosuppression in the tumor microenvironment. All of this has an effect on tumor-reactive CD8 T cells, increases proinflammatory cytokines, and decreases transforming growth factor-B (TGF-B), among other immunosuppressive molecules, and weakens T-cell immunosuppression, Importantly, There was "improved lung tumor regression and long-term survival in mice. Respiratory hyperoxia also promoted the regression of spontaneous metastasis from orthotopically grown breast tumors." The researchers noted that these results are "entirely T cell-and natural killer cell-dependent, thereby justifying the testing of supplemental oxygen as an immunological coadjuvant to combine with existing immunotherapy for cancer."
*SOME OF THE PROBLEMS WITH LOW OXYGEN (HYPOXIA) AND THE BENEFITS OF ADDING OXYGEN HAVE JUST BEEN DESCRIBED.
RETURNING TO OZONE WITH ITS ADDITIONAL ATOM OF OXYGEN - O3
OZONE THERAPY - OZONE BACKGROUND
"Ozone therapy has been in use since the 1800s and, in 1896, the genius Nikola Tesla patented the first O3 generator in the US, later forming the "Tesla Ozone Company". During the first world war (1914-18) doctors familiar with O3's antibacterial properties, and with few other medical resources available to them, applied it topically to infected wounds and discovered O3 not only remedied infection, but also had hemodynamic and anti-inflammatory properties. In the late 1980s, reports had emerged that German physicians were successfully treating HIV patients with O3-AHT (autohemotherapy)." . AM Elvis and JS Ekta, 2011.
"In the scientific literature, the first mention of ozone was made by the Dutch physicist Martin van Marum in 1785. During experiments with a powerful electrification installation, he discovered that by passing an electric spark through the air, a gaseous substance with a characteristic odor appeared that has strong oxidizing properties. In 1840, the University of Basel professor, Christian Friedrich Schonbein linked the information on the changes of the properties of oxygen with the formation of a particular gas that he called ozone (from the Greek word, ozein, "to smell".) A. Schwartz, MD and G Sanchez, PHD, 20
OZONE THERAPY - WHAT IS OZONE?
" Ozone in air pollution is AIR POLLUTION. It is also a reflection of many volatile toxic organic compounds in air pollution which are highly toxic. No one would quarrel that air pollution is toxic. We do not administer air pollution medically. We administer ozone generated via medical grade oxygen (100%), so it is free of noxious organic pollutants which ozone in smog reflects It is NOT administered into the lungs as is pollution, but given in any number of methods to other parts of the body or blood." Robert Rowen, MD, 2015.
"Ozone is a reactive form of oxygen that exists naturally as a trace gas in the atmosphere. It is perhaps best known for its crucial role absorbing ultraviolet radiation in the stratosphere, where it is concentrated in a so-called ozone layer - protecting life on earth from solar radiation." See https://www.scripps.edu/news/press/2003/022703.html.) "The oxygen you breathe is present in the air as a pair of oxygen atoms. This is the most stable form of oxygen, and it's colorless. Ozone is a blue colored form of oxygen (it's what makes the sky blue), and unlike regular oxygen, it is composed of three oxygen atoms instead of two. It is the addition of the third oxygen atom that makes ozone 'supercharged' oxygen, and gives it all of its remarkable medical properties." https://www.antiagingmedicine.com/treatments/ozone-therapy
*THERE ARE MANY DIFFERENT WAYS TO USE OZONE. AT THE BEGINNING OF THIS SECTION ARE BLOOD SAMPLES FROM MAH (MAJOR AUTOHEMOTHERAPY). THERE IS DRINKING OZONATED WATER, BAGGING, AND RECTAL AND VAGINAL INSUFFLATION. BACK IN THE SIXTIES, ROBERT ATKINS, MD, REPORTEDLY TOLD HIS RADIO AUDIENCE THAT HE INJECTED OZONE DIRECTLY INTO A BREAST TUMOR AND CURED THE PATIENT. (HE LOST HIS LICENSE UNTIL HE PROMISED TO NEVER AGAIN DISCUSS OZONE.)
JOHANN LAHODNY, MD, PHD'S NEW HIGH DOSE OZONE TECHNIQUE - MAH (MAJOR AUTOHEMOTHERAPY): "10 PASSES OF BLOOD, 200 CC EACH PASS, 70 GAMMA EACH TIME. TOTAL AMOUNT OF OZONE IS 140,000 MCG" - 10 PASS FOR CANCER "Complementary therapy in all cancers. Through direct tumoral-inhibiting effect of oxygen-ozone mixtures, by anti-tumor effect of oxygen-induced interferon production by enormous increase in immune cells and enzyme release. High doses of ozone are able to reduce both primary cancer tumors and cancer metastasis. You can achieve amazing success in late stage cancer."
"1. Ozone therapy improves red cell flexibility, meaning your blood will flow much better. RBCs (red blood cells) will be able to flex better to get through the small capillaries. They will carry a higher negative electrical charge so that they repel each other and don’t stack together. This improves rheological (flow) properties of your blood, enabling more oxygen transport.
2. Ozone therapy stimulates your RBCs to generate a compound called 2,3 DGP. Interestingly, this molecule enables your RBCs to release their payload of oxygen into your tissues. Less 2,3 DGP and your RBCs might actually hold on to the payload leaving your tissues to become oxygen starved.
3. Ozone therapy increases RBC ATP. The increased energy in the red cells enable newly created RBCs to become 'supergifted' in the words of researcher Velio Bocci, MD of Italy.
4. Ozone therapy appears to turn on overall mitochondrial function in your cells. Mitochondria are your cell furnaces where energy is made. More energy, more ability to repair, no matter the tissue or organ. This conclusion is based on basic science research of German and Cuban researchers showing more oxygen consumption in tissues.
5. Researchers Bocci in Italy and Silvia Menendez, PhD and her team in Cuba have determined that ozone modulates the immune system. In other words, where there is inflammation that is not needed, ozone will dampen it, and allow inflamed tissues to heal. Where the immune system is weak, ozone therapy picks it up. The net effect of ozone therapy is to bring your immune system to a healthier set point of balance. You need inflammation to fight invaders and repair, but when inflammation is not turned off, the inflammation itself then becomes destructive to your tissues. In the case of joints, modulating inflammation with ozone can sometimes lead to instant results 6. Ozone and its metabolic products are directly toxic to pathogens. In fact ozone virtually instantly punches holes in the membranes of bacteria hemorrhaging them on the spot. In contrast, chlorine compounds, well known as disinfectants, are 100x slower!" (See Robert Rowen, MD, https://www.facebook.com/DrRobertJRowen/, 2015.)
OZONE THERAPY - CAN OZONE KILL BACTERIA?
"According to microbiological research data, the ozone is capable of killing all the known types of gram-positive and gram-negative bacteria, including the Pseudomona aeruginosa and Eschericea coli; both bacteria are tremendously resistant to antibiotics. The local disinfectant, antiviral and antibacterial effects of ozone, therefore, are due to its germicide capacity, basically to its high oxidant capacity on the bacterial walls. This fact makes it a general broad spectrum germicide on which the classic mechanisms of microbial resistance do not act."
(See Sheetal P et al., The Power of Small Changes: Comprehensive Analyses of Microbial Dysbiosis in Breast Cancer, BBA, 2019. The power of small changes: Comprehensive analyses of microbial dysbiosis in breast cancer Sheetal P
In their 2019 Review Article, James Lawson and Wendy Glenn found DNA gene sequences, which are almost identical to the mouse tumor virus, in up to 40% of human breast cancers. These mouse-like gene sequences are up to 15-fold more prevalent in human breast cancer than in benign tumors or normal tissues. In fact, these sequences have been found from 1-11 years in normal and benign breast tissues prior to the onset of mouse-like viruses in breast cancer. Can ozone kill this mouse-like virus?.... And how about a 2015 study that found that 59% of breast cancer tumors harbor the bovine leukemia virus. Can ozone kill this delta retrovirus? In addition, what about the "distinct biological signatures" found in a 2015 study on triple negative breast cancer? Can ozone kill the herpes viruses, fungal families, bacteria, and parasites that defined a hierarchy in triple negative breast cancer. ... Plus, Hulda Clark, PHD, found not just a parasite, Fasciolopsis buski, but a myriad viruses and bacteria contributing to cancer. Can ozone kill all of these pathogens?
OZONE THERAPY - CAN OZONE KILL VIRUSES?
Robert Rowen, MD, wrote, "When it comes to viruses, these stealth pathogens MUST gain entry to your cells to wreak their havoc, no matter the virus. Research has shown that most viruses (at least every one I’ve looked at) require fully functional and reduced sulfhydryl groups (SH) on their outer shell to attach to and enter your cells. The SH groups are the 'fingers' viruses use to open and enter your cells according to world literature.Ozone is extremely reactive oxygen.
It will strip the H off the SH as follows: SH + SH +O3 > S-S = H2O + O2. This is called 'oxidation'. The only residual product is oxygen, which your body burns, and water, so no toxicity whatsoever! If the SH groups are oxidized to S-S, the virus is inactivated. It can’t enter your cells. And that is the best thing that could happen for you. See, if the virus is present in your blood stream, but can’t enter your cells, your immune system can see them without the tissue destruction. This should enable you to mount a proper and fast immune defense and dispatch the invader without damage to you! .... I thought ozone would be a good match for Ebola. Research has shown that Ebola has the same 'Achilles Heel' as other viruses, in that it has glycoproteins (proteins with sugar attached) on its surface, which have sulfur groups that MUST be reduced to SH in order to gain cell entry."
THE HUMAN BODY MAKES OZONE! REALLY!! FIRST WAY!! ANTIBODIES ATTACH TO NEUTROPHILS (THAT KILL BACTERIA). NEUTROPHILS GIVE SINGLET OXYGEN TO ANTIBODIES > ANTIBODIES CONVERT SINGLET OXYGEN TO OZONE TO HELP NEUTROPHILS KILL MORE BACTERIA!!
" ...In human neutrophils: Antibodies generate both hydrogen peroxide and ozone during the water-oxidation pathway which are bactericidal in combination and which makes immunoglobulins the third enzyme in the microbicidal pathway of neutrophils, after the phagocyte oxidase and myeloperoxidase . Inset is an electron micrograph of an E. coli bacterium killed by the antibody-catalyzed water-oxidation pathway. There is remarkable simile in the morphology of cell wall and membrane rupture to that of when bacteria are phagocytosed."
So, how does the body make ozone? One way, via antibodies and neutrophils...
Antibodies are proteins that detect foreign antigens, such as bacteria, parasites, and fungi. Neutrophils are a type of white blood cell that importantly fight infections. From a Scripps Research Institute 2003 Press Release: " ...For the last 100 years, immunologists believed that antibodies - proteins secreted into the blood by the immune system - acted only to recognize foreign pathogens and attract lethal 'effector' immune cells to the site of infection."
But Scripps researchers in 2003 found a novel role for our bodies' antibodies.
Richard Lerner, MD, and Paul Wentworth, PHD, "demonstrated that antibodies are able to produce ozone and other chemical oxidants when they are fed a reactive form of oxygen called singlet oxygen". And, in 2004, it was discovered that the "oxidants produced by antibodies can destroy bacteria by poking holes in their cell walls."
But, how do antibodies make ozone?
"The Scripps research team knew that in order to make the ozone and other highly reactive oxidants, the antibodies had to use a starting material known as singlet oxygen, a rare, excited form of oxygen." And the researchers believed that they found the answer, where the singlet oxygen was derived from.
From Babior BM et al., 2003, "Given that neutrophils have the potential both to produce singlet oxygen and to bind antibodies, we considered that these cells could be a biological source of ozone. We report here further analytical evidence that antibody-coated neturophils, after activation, produce an oxidant with the chemical signature of ozone." The singlet oxygen comes from neutrophils, which are "one of the effector immune cells ... which are little cellular factories that produce singlet oxygen and other oxidants. During an immune response, the neutrophils engulf and destroy bacteria and fungi by blasting them with these oxidants."
From the Scripps Press Release: "The work of the Scripps scientists suggests that the antibacterial effect of neutrophils is enhanced by antibodies. In addition to killing the bacteria themselves, the neutrophils feed single oxygen to the antibodies, which convert it into ozone, adding weapons to the arsenal."
THE HUMAN BODY MAKES OZONE PLUS OTHER OXIDANTS - REALLY!! - OZONE + HYDROGEN PEROXIDE = H2O3 (DIHYDROGENTRIOXIDE)
Jason Socrates Bardi, in a 2004 Scripps Research Institute article, reported the detection of "the elusive dihydrogen trioxide in a reaction between ozone and hydrogen peroxide". Dihydrogen trioxide is a "short-lived and powerful intermediate molecule".
"... Because ozone and hydrogen peroxide may both be found in vivo (in the human body) as part of an immune process whereby the body produces oxidants in order to kill invading microorganisms. Since ozone and hydrogen peroxide mixed together can form dihydrogen trioxide, and since ozone and hydrogen peroxide can be found in living systems, dihydrogen trioxide may also be a part of biology,"
As background, when test tube antibody experiments found that singlet oxygen, "during normal metabolic processes" could form hydrogen peroxide, the thought was that antibodies could do more than just surveil the bad guys, like bacteria, since oxidants kill pathogens.
But, "The amount of hydrogen peroxide the antibodies generated was not enough to kill the number of bacteria they observed dying. So perhaps the antibodies were producing other oxidative species as well." And that is when the researchers discovered the OZONE signature.
And, going back to dihydrogen trioxide...
"Since ozone and hydrogen peroxide are both part of biological systems, and since dihydrogen trioxide has now been detected in the reaction of hydrogen peroxide and ozone, it stands to reason, says Paul Wentworth,PHD, (a Scripps researcher) that the powerful oxidant dihydrogen trioxide may be a biologically relevant molecule present in biological systems and acting as an oxidant in the immune system’s reaction against bacteria."
THE BODY MAKES OZONE! REALLY!! -SECOND WAY! VIA 4 AMINO ACIDS AMINO ACIDS > SINGLET OXYGEN>OZONE>TO HELP NEUTROPHILS
In the 2008 Yamashita K et al. study, the researchers "discovered that 4 amino acids themselves were able to catalyze the production of an oxidant with the chemical signature of ozone from singlet oxygen in the water -oxidation pathway, at comparable levels to antibodies."
The researchers commented, "Previous observation revealed that antibody catalyzed ozone formation by human neutrophils contributed to the killing of bacteria." (SEE BABIOR BM ET AL. STUDY ABOVE.)
Thus, the researchers do"favor the proposal ... that antibodies can catalyze ozone generation in neturophils. But they go further...
"Our results further suggested the hypothesis that amino acids themselves exhibited catalytic activity to convert singlet oxyghen and water to an oxidant with the chemical signature of ozone, and amino acid-catalyzed oxidant with the chemical signature of ozone showed bactericidal activity in human neutrophils." ... In human neutrophils we examined, the bactericidal activity induced by the addition of amino acids was prominent when larger numbers of E.coli were added to neutrophils... Thus, ozone produced by neutrophils might potentiate a host defense system when the host is challenged by high doses of infectious agents."
Conclusion: Thus, "The resultant oxidant with the chemical signature of ozone exhibited significant bactericidal activity in our distinct cell-free system and in human neutrophils. The results also suggest than an oxidant with the chemical signature of ozone produced by neutrophils might potentiate a host defense system, when the host is challenged by high doses of infectious agents. Our findings provide biological insights into the killing of bacteria by neutrophils."
(See Yamashita K et al, Ozone Production by Amino Acids Contribute to Killing of Bacteria, PNAS, 2008, www.pnas.org/content/105/44/16912.full 2008 )
"OZONE THERAPY FOR BREAST CANCER: IT JUST MAKES COMMON SENSE"
Veronique Desaulniers, DC, believes that ozone therapy "makes sense" for breast cancer patients.
We have shown in this OZONE THERAPY section that when there are low oxygen levels in tumors that more proteins are made that promote tumor growth. More oxygen is needed!
Veronique Desaulniers, DC, wrote, "In 1966, Otto Warburg, a Nobel Prize-winning doctor from Germany, put the relationship between cancer and oxygen this way: 'Cancer cells meet their energy needs by fermentation, not oxidation. Thus, they are dependent upon glucose and a high oxygen environment is toxic to them.' "
Thus, "Based on this statement alone, it just makes sense that a healing modality that infuses internal environments with oxygen might shrink tumors and help heal cancer. And in the simplest terms, this is exactly what ozone therapy (and other oxygen-depended therapies like hydrogen peroxide) may do- with extremely positive results."
She concluded, "When it (ozone) is used in conjunction with a healing diet and other modalities ..., the fact that ozone's super-oxygenating qualities might stimulate and modulate the immune system, clear the blood, reduce tumors, and help heal the body overall, is a real no-brainer."
OZONE THERAPY - 1980 OZONE-CANCER CELL STUDY - HOW SWEET IS THE SWEET STUDY ON OZONE AND CANCER CELLS, INCLUDING BREAST CANCER CELLS!!
"In 1980, the journal Science published Ozone Selectively Inhibits the Growth of Human Cancer Cells. The study showed that ozone inhibits the growth of human lung cancer, breast, and uterine cancer cells." Cited below is the full 1980 study:
Way back in 1980 ... breast cancer cells and other cancer cells were treated with ozone...
In the Sweet F et al. study, cancer cells were "selectively inhibited in a dose-dependent manner by ozone at 0.3 to 0.8 part per million of ozone in ambient air during 8 days of culture".
Results: "The presence of ozone at 0.3 to 0.5 part per million inhibited cancer cell growth 40 and 60 percent, respectively." Healthy lung cells were unaffected. Conclusion: "Exposure to ozone at 0.8 part per million inhibited cancer cell growth more than 90 percent and control cell growth less than 50 percent. Evidently, the mechanisms for defense against ozone damage are impaired in human cancer cells."
(See Sweet F et al., Ozone Selectively Inhibits Growth of Human Cancer Cells, Science, 1980. To reiterate, for the full study: https://abo3vet.com.br/wp-content/uploads/2018/12/Ozone-Selectively-inhibits-growth-of-human-cancer-cells.pdf#page=1&zoom=auto,-265,681)
OZONE THERAPY - 2015 STUDY- OZONATED WATER DIRECTLY ADMINISTERED INTO TUMOR-BEARING MOUSE MODEL RESULTED IN NECROSIS AND INHIBITED PROLIFERATION OF TUMOR CELLS
NOTE THAT NT ARE THE NOT TREATED TUMORS, WHILE THE CONTROL GROUP RECEIVED DISTILLED WATER, AND THE O3 - OZONATED WATER - TUMORS SHOWED THE HIGHEST RATE OF NECROSIS.
In the Kuroda K et al. 2015 study, the researchers "conducted a study on the safety of ozonated water and its anti-tumor effects using a tumor-bearing mouse model and normal controls."
" ....Local administration of ozonated water (20.8, 41.6, 104, or 208 mM) directly into the tumor tissue induced necrosis and inhibited proliferation of tumor cells. ... The size of the necrotic areas was dependent on the concentration of ozonated water."
How does ozonated water work? "There is a possibility that it exerts (its effect) through the production of reaction oxygen species (ROS). ...Many tumor cells have few antioxidant materials, such as superoxide dismutase (SOD), catalase, and glutathione peroxidase, in comparison with normal cells. Therefore, the tumor cells are greatly affected by reactive oxygen species (ROS). ... ROS can interact with numerous cellular components, including DNA, lipids, and proteins. The consequences of these interactions include losses of cell integrity, enzyme function, and genomic stability. When ozone is administered in vivo, it is well know that ROS, such as hydrogen peroxide (H2O2), are generated. Therefore, the anti-tumor mechanism of ozonated water is likely to also be the cell injury caused by ROS." (EDITORS' NOTE: Some chemo drugs and high dose vit. C produce ROS.)
Import of necrosis? "In a previous report, a decrease in metastasis and an extension in the duration of survival were observed in a mouse tumor necrosis model compared to a mouse tumor apoptosis model. Therefore the induction of necrosis rather than apoptosis by administration of ozonated water is very useful in tumor immunity."
Conclusion: "In this study, it is suggested that ozonated water has an anti-tumor effect. There is a possibility that it is exerted through the production of ROS. Moreover, ozonated water has low toxicity and is safe and easy to handle." Plus, there weren't any "detrimental effects in normal tissues". Therefore, "We believe that administration of ozonated water is a safe and potentially simple adjunct or alternative to existing antineoplastic treatments."
(See Kuroda K et al., The Safety and Anti-Tumor Effects of Ozonated Water in Vivo, Int J Mol Sci, 2015.)
BACK IN 1998 - OZONE PRECONDITIONING BEFORE GIVING CARBON TETRACHLORIDE, A POISON, TO RATS! "OZONE COMES TO TYPIFY A UNIQUE DRUG."
In the Leon OS et al. 1998 study, the researchers, in an effort "to demonstrate the capability of O3 (ozone) to promote an oxidative pre-conditioning process, introduced hepatocellular (liver) damage with a single dose of carbon tetrachloride (CC14), which is a recognized organic agent able to produce cellular injury through generation of free radicals."
Repeated oxygen-ozone administration "via colorectal route can induce a sort of cross tolerance to free radicals released after one single dose of carbon tetrachloride. On the contrary, experimental groups treated with either carbon tetrachloride or oxygen + carbon tetrachloride, respectively, displayed significant cellular damage".
Conclusion: The tolerance to both ozone and ROS (reactive oxygen species) must be "demoninated as either 'oxidative pre-conditioning'. or 'oxidative stress adaptation'. Ozone therapy has been able to preserve liver integrity by inducing either enzymes or activating metabolic pathways that maintain an equilibrated redox balance. High SODs and GSH levels, low peroxidation, and a normal Ca2+ (calcium) homeostatis are clear examples of the efficacy of ozone therapy. We believe that the possibility shown by appropriate ozone therapy to upregulate the antioxidant system represents a fundamental property of this complementary medical approach.... Antioxidant substances such as ascorbic acid, GSH, n-acetylcysteine, SOD, and the like are useful but hardly able to dramatically reverse a chronic oxidative stress. In this sense, the improvement of the antixidant defense is bound to be crucial in cancer, chronic viral infections, and neurodenegerative diseases where the control of endogenous oxidation has gone awry with progressive cell damage." (See Leon OS et al., Ozone Oxidative Preconditioning: A Protection Against Cellular Damage by Free Radicals, Mediators of Inflammation, 1998.)
* HAVING RADIATION THERAPY? CT SCANS? HAVING ANY RADIATION AT ALL? OZONE PRE-CONDITIONING TO PREVENT DAMAGE?
OZONE THERAPY - 2013 STUDY: CAN OZONE BEFORE RADIATION AMELIORATE DAMAGE?
Yes!..... According to a 2013 study, pre-conditioning with ozone is a radioprotector!
"Because radiation-induced cellular damage is attributed primarily to harmful effects of free radicals, molecules with direct free radical scavenging properties are particularly promising as radioprotectors."
And the Gultekin FA et al. researchers, knowing that "controlled ozone administration may promote an adaptation to oxidative stress, preventing the damage induced by ROS, .. hypothesized that ozone would ameliorate oxidative damage caused by total body irradiation with a single dose of 6gy in rat liver and ileum (small intestine) tissues."
What the researchers were experimenting with is called "pre-conditioning". In a terrific article, Frank Shallenberger, MD, discussed pre-conditioning with ozone as elaborated in this study. "Pre-conditioning is when people are treated before they get sick in order to prevent them from becoming sick. Wow, what a concept! The researchers knew that radiation damages tissues through the action of free radicals. They also knew that the most effective way to combat free radical damage is by using ozone therapy. And so they did a very simple thing. They pre-conditioned a group of rats with ozone therapy for five days. Then they exposed them and an unlucky group of rats that had no ozone pre-conditioning to a single whole body dose of 6Gy (Grey units) of radiation. This is a huge exposure. Keep in mind that acute radiation poisoning happens at doses over 0.1 Gy. This dose was 60 times the upper limit! After the exposure, the researchers measured the effects of the radiation in al of the rats."
Results? Dr. Shallenberger commented, "Not surprisingly the radiation in the untreated rats caused a significant increase in the levels of free radicals and a significant decrease in the levels of the enzymes, such as SOD (superoxide dismutase), which the body used to protect itself from free radical damage. And what happened to our little pre-conditioned critters? They did much better. In the words of the authors, 'The results demonstrated that the levels of free radicals in liver and ileal tissues significantly increased'. But that's not even the most amazing part of the study."
Here is the "amazing" part of the study.... "When they looked at the actual radiation damage that happened to the livers and small intestines of the pre-conditioned rats, the ozone 'reversed all histo-pathological alterations induced by irradiation'. This means that there were absolutely no signs of any damage at all in any of the pre-conditioned rats. This is simply astonishing."
Conclusion: From the study, "In conclusion, data obtained from this study indicated that ozone could increase the endogenous antioxidant defense mechanism in rats and thereby protect the animals from radiation-induced organ toxicity."
* Dr. Shallenberger posed the question: "And why is this so important? One reason is obvious. If you have cancer and are regularly getting radiated with either CT scans or radiation therapy, you can protect your body from all of the negative effects by pre-conditioning with ozone."
Further, "But will the ozone therapy some how protect the cancer cells in the same way it protects healthy cells? Absolutely not. This is because, as this study clearly shows, ozone exerts its protective effects by stimulating antioxidant enzymes such as SOD. And while healthy cells can make these enzymes, the beautiful thing is that cancer cells can't! In fact, the added oxidant stress from ozone therapy only serves to make cancer cells more vulnerable to radiation. I routinely use ozone therapy in every single one of my cancer patients because of this remarkable effect." (For the article, see Frank Shallenberger, MD, Pre-Conditioning is a Form of Therapy That's in the Process of Completely Changing the Face of Preventive Medicine, 2015. ... For the study, see Gultekin FA et al., Effects of Ozone Oxidative Pre-Conditioning on Radiation-Induced Organ Damage in Rats, J Radiat Res, 2013.)
OZONE THERAPY - BISPHOSONATES FOR BREAST CANCER, THEN DO DENTAL WORK, WITH POSSIBLE OSTEONECROSIS OF THE JAW LESION OZONE THERAPY RESPONSE RATE IN OSTEONECROSIS OF THE JAW IS 75%!
Osteonecrosis of the jaw may occur in breast cancer and other cancer patients receiving bisphosphonates (antiresorptive drugs aiming to slow or stop the dissolving of bone tissue) and denosumab.
In the Ripamonti CI et al. 2012 study, the researchers evaluated the effects of ozone on patients who had been treated with the bisphosphonate, zoledronic acid, and developed osteonecrosis jaw lesions >2.5 cm.
There is always a chance that breast cancer patients taking bisphosphonates can develop osteonecrosis of the jaw.
The shared complication of osteonecrosis of the jaw in cancer patients treated with high doses of denosumab or potent bisphosphonates strongly suggests that profound and prolonged inhibition of bone resorption with oversuppression of bone remodeling is the primary mechanism. Animal data support the view that a higher rate of bone remodeling or more severe oversuppression of the bone turnover rate with bisphosphonates in the jawbones may explain the differential predisposition at this site compared to other bones in the axial or appendicular skeleton." In addition, in this study, zoledronic acid was used. Note that, "Medically induced ostenecrosis of the jaw is a form of avascular necrosis, and thus not surprisingly, inhibition of angiogenesis is another leading hypothesis. In vitro experiments consistently demonstrate that zoledronic acid inhibits angiogenesis, and cancer patients treated with this agent have decreased circulating VEGf levels." (See http://www.uptodate.com/contents/medication-related-osteonecrosis-of-the-jaw-in-patients-with-cancer.)
In this study, specifically, 24 patients, including 11 patients with breast cancer that had metastasized to the bone, who hadn't had any benefits from standard therapy, were treated with medical ozone treatment "delivered as gas insufflations on each osteonecrosis jaw lesion". The ozone treatment was given "every third day for a minimum of 10 for each pathological area or till necrotic bone sequestrum (dead bone tissue) or surgery. In 12 patients with the largest and deeper lesions, O 3 gas therapy produced the sequestrum of the necrotic bone after 10 to 38 insufflations; surgery was necessary to remove it (11 patients). Of interest, removal was possible without the resection of healthy mandible edge because of the presence of bone sequestrum. All together the response rate was 75.0% In all patients treated with O 3 gas ± surgery, no osteonecrosis of the jaw relapse appeared (follow-up mean 18 months, range 1–3 years).
Conclusion: This study "shows that the topical insufflation of ozone medical gas on osteonecrosis jaw lesions >2.5 cm is able to produce a sequestrum of the necrotic bone with 1. its spontaneous or not traumatic explusion and the healing of the gum mucosa followed by re-epithelialization 2. or surgical removal of the necrotic bone and healing of the gum mucosa. This is an effective and original method, in the absence of observable toxicity. Thus, medical O 3 gas insufflations appears to be an effective and safe treatment for patients treated with bisphosphonates who developed ostenecrosis jaw lesions >2.5 cm".
(See Ripamonti CI et al., Efficacy and Tolerability of Medical Ozone Gas Insufflations in Patients with Osteonecrosis of the Jaw Treated with Bisphosphonates - Preliminary Data, Journal of Bone Oncology, 2012.)
OZONE THERAPY - CASE REPORT: PERSISTENT FISTULA IN IRRADIATED AREA IN A BREAST CANCER PATIENT UNRESPONSIVE TO TREATMENT.SUCCESSFULLY TREATED WITH LOCAL OZONE APPLICATION
Changes in "pre (upper)- and post (lower)-ozone treatment provided more objective assessment of the changes than palpation i.e. decrease in thickness and dimensions of the induration/fibrosis area."
Definition of a fistula: "A fistula is an abnormal connection between an organ, vessel, or intestine, and another strucure. Fistulas are usually the result of injury or surgery. It can also result from infection or inflammation." (See https://www.nlm.nih.gov/.../0.)
"Persistent and delayed healing of fistulae and local infection in previously irradiated areas can increase the risk of systemic complications and complicated clinical management." And, of course, cancer patients have impaired immune systems
There was a case of a persistent PORT-a-CATH A-related fistula and fibrosis in a breast cancer patient.
What can be done?
Surgery can have side effects along with delayed healing. Hyperbaric oxygen is "cumbersome" and not always available.
So, the Clavo B et al. researchers, in 2012, decided to use ozone!
Conclusion: These researchers "described a case of a persistent fistula in a previously irradiated area, which was refractory to treatment but which was successfully treated with local ozone applications"
. (See Clavo B et al., Persistent PORT-a-CATH Â®-Related Fistula and Fibrosis in a Breast Cancer Patient Successfully Treated with Local Ozone Application, Journal of Pain and Symptom Management, 2012.)
OZONE THERAPY - BREAST CANCER-RELATED LYMPHEDEMA -RATE OF THICKNESS REDUCTION IN ARMS WAS MORE OBSERVABLE AFTER OZONE THERAPY
In the 2013 Waked SI et al. study, the researchers sought "to evaluate the efficacy of ozone therapy in breast cancer-related lymphedema".
Background on lymphedema: "Breast cancer-related lymphedema is a serious condition that often causes pain and disability and can predispose patients to life-threatening complications, such as, infection, limitation of function, loss of body image and self-esteem, affective disorders and fear. Physical morbidity ... includes decreased shoulder mobility and neural tissue injury causing sensory and motor dysfunction. All lead to diminished quality of life and limitations in activities of daily living of the upper limbs."
The researchers decided to use an oxidative therapy, namely ozone, to combat lymphedema.
"Recent research suggests that lymphedema might be improved by high-pressure oxygen therapy. The use of oxygen might stimulate the growth of new lymphatic channels and reduce the scar tissues surrounding existing lymphatic channels leading to reduction of limb volume. ....Ozone is a naturally-occurring configuration of three oxygen atoms ... Ozone manifests bactericidal, virucidal and fungicidal actions that might make it a treatment of choice in certain conditions and an adjunctive treatment in others. Ozone reacts with blood generates pharmacological messengers such as hydrogene peroxide, and lipid oxidation products, these activate several biochemical pathways in blood cells, which are responsible for therapeutic activities lasting several days. The result is improved red blood cell oxygen delivery, activated white blood cell immune response, platelet release of growth factors, endothelial release of nitrous oxide, release of stem cells from the bone morrow, and the upregulation of antioxidant enzymes in the various organs. Some recent studies demonstrated that, ozone significantly increases the amount of platelet-derived growth factor, transforming growth factor b1, ,and interleukin-8. These factors are released in a dose-dependent manner and are considered as proinflammatory and enhance angiogenesis of lymphatic vessels."
In this study, sixty breast cancer patients with lymphedema were placed in two groups: "The ozone group received ozone therapy in addition to complex physical therapy for lymphedema consisted of pressure garments, remedial exercise, and skin care, while the control group received complex physical therapy only." The ozone group received their ozone via rectal insufflation, up to 32 treatments in twelve weeks.
Results: While pretty comparable physically at the start of the experiment, the two groups similarly had a reduction in limb volume at twelve weeks. However, "The trend was more significantly pronounced in ozone group than the control group. There was significant reduction in skin and subcutis thickness in both groups. The rate of thickness reduction was more observable in the ozone group than the control group."
Conclusion: ""Ozone may be clinically effective in reducing limb volume and subcutaneous thickness in patients with breast cancer-related lymphedema. So ozone therapy could be considered as an adjunct therapy in addition to gold standard therapy that includes manual lymph drainage, compression garments, and skin care in the treatment of women with breast cancer-related lymphedema."
(See Waked SI et al., A Single Blinded Randomized Controlled Clinical Trial on the Efficacy of Ozone Therapy on Breast Cancer-Related Lymphedema, www.ccsenet.org/cco Cancer and Clinical Oncology Vol. 2, No. 2; 2013.)
OZONE THERAPY - LOTS OF WAYS TO OZONATE - CONSULT WITH A MEDICAL PRACTITIONER -HERE IS ONE WAY TO OZONATE - WHOLE BODY OZONATION - HULDA CLARK, PHD'S INSTRUCTIONS EDITORS' NOTE: THIS METHOD USES AN OZONATOR, NOT WITH MEDICAL OXYGEN. THUS, ONLY AMBIENT AIR IS USED. HOWEVER, MOST OZONE PRACTITIONERS DO USE MEDICAL OXYGEN TO OZONATE.
" Purchase large plastic bags (50 gallon or larger) meant for leaves or trash. You should be able to stand in one and have it come just over the waist. Also purchase a roll of 1/2 inch masking tape (tan color). Your ozonator should have been tested for nickel emission even though you will NOT breathe it and also have black rubber tubing replaced.
Ozonate after a hot shower. The shower is not essential. This lets the ozone penetrate the skin, without you getting chilled. Place the ozonator on the floor beside a comfortable chair. Set the tape, scissors, twist ties beside you. It is not necessary to undress.
Cut a small hole, just big enough to let the ozonator hose through the bag, about half way up the front. Tape the hose tightly to the bag so no ozone can escape there. Leave it attaches; no need to take it apart each day. You should NOT be able to smell the ozone.
Standing in the bag, tighten it around your upper waist; then twist it. Fold it down. Do not tape to skin. Sit down. Turn on the ozonator. The bag will begin to puff out in 5 or 10 minutes. It must puff out to show you there is pressure inside.
Change position a lot to stay comfortable. Sitting on the edge of the chair or leaning back does not make a difference.
If your illness is very advanced, try to ozonate for 1 1/2 to 2 hours, 2 or 3 times a day. It will kill parasites, bacteria, and viruses altogether without side effects.
After turning off the ozonator and getting out, turn the bag upside down to let any sweat or moisture drain. Change the bag if you sweated in it, or turn it inside out. Push the bag aside so you don't breathe the stale ozone. Take another hot shower right after the ozonation to prevent reabsorbing your sweat." (See Hulda Clark, PHD, New Concepts, Page 17.) (Editors' Note: For ozone bags, consider Kirkland's 55 Gallon Drum Liners, from Costco. Also, consider ozone bagging of the affected breast.)
EDITORS' NOTE: TO REITERATE, THERE ARE MANY WAYS TO USE OZONE. PLEASE CONSULT WITH A KNOWLEDGEABLE MEDICAL PROFESSIONAL BEFORE PURCHASING OR USING ANY OZONE DEVICE! __________________________